Removal of crude oil by Thiobacillus sp. and Clostridium sp. at various temperatures and concentration of pollutant in liquid media

Abstract The activities of the petroleum mining industry in Indonesia have caused many problems by producing hazardous waste pollution that would produce dangers for plants, animals or human lives. One technique to recover environmental situation is through land farming technique. purpose this study overcome crude oil environment using biotechnology method process enzymatic bioremediation. parameters discussed determine optimal conditions provide highest efficiency Total Petroleum Hydrocarbon (TPH) degradation processes such as temperature with a variation 25 – 45°C then concentration pollutants 5% 20%, and contact time carried out solid media variations amounting 3 21 days. After conducting research, research produces bacterium Thiobacillus sp. Clostridium under at 35°C pollutant 10% 14 Thus, it can be concluded set aside hydrocarbon compounds removal 65%. So, needs done other physical parameters.